confocal
Microscopes

Olympus has developed two confocal designs to meet the challenging needs of high-end researchers. Our FluoView™ Laser Scanning Confocal Series offers a choice of two models for a variety of applications requiring ultra-thin sectioning. Our Spinning Disk Confocal system allows high transmission rates and greater acquisition speed through a rotating slit aperture for reduced phototoxicity. Both designs are compatible with the Olympus research range of microscopes offering high-resolution confocal optical sectioning.

FluoView™ FV1000 MPE

Multiphoton Microscope

A Multiphoton* Laser Scanning Microscope excellent for deep tissue imaging.

Olympus' Fluoview FV1000-MPE, a multiphoton laser scanning microscope that allows fluorescence imaging deep within specimens. Utilizing pulsed IR lasers, the Olympus Fluoview MPE is able to image hundreds of microns into a specimen thanks to the penetration of IR light and our long working distance objectives. The Fluoview-MPE provides cutting-edge technology for various areas of scientific research such as neuroscience and cell biology.

Features and Benefits

Optimal for in-vivo observation requiring deeper imaging.

• Reduced unwanted photobleaching via excitation only in the focal plane.
• UIS2 optical system designed for multiphoton* with long working distances and improved infrared transmission.
• Efficient acquisition of fluorescence emission using non-descanned photomultipliers located close to the objective
• Excellent for patch clamp techniques using our BX61WI Microscope frame
• Beam conditioning with our Negative Chirp optical system allows researchers to image deeper into the specimen than previously possible. Image below is of rat cerebral cortex section over 600 microns thick

Simultaneous Two-photon Imaging and Two-photon Stimulation

Our exclusive FluoView SIM scanner technology provides two scanning modules that can both be equipped with a two-photon laser offering the ability to simultaneously image and photoactivate/stimulate two distinct areas of a specimen. Our propietary SIM dual scanner technology is unique to our FV1000 single and multiphoton systems.

FV1000-MPE System Configurations and Capabilities

Basic

• Attenuation via 1/2 wave plate + Glen Thompson Prism
• Switching speed 10ms (mechanical shutter )
• Simple optical system
• 2-Channel Non-Descanned Detector

Designed for the researcher who wants to conduct an experiment using Region of Interests

• Attenuation via our AOM (Acoustic Optical Modulator)
• Switching speed 10usec
• ROI scanning possible
• Automatic blinking
• Negative Chirp beam conditioning optics for high efficiency 2 photon excitation
• 2-Channel Non-Descanned Detector

Designed for the researcher that needs the ultimate in multiphoton microscopy.

• Attenuation: AOM (A.A.) for Imaging Scan Unit and SIM scanner
• Negative Chirp beam conditioning for both Imaging & SIM lasers
• High efficiency 2 photon excitation
• IR pulse laser for SIM scanner
• Two photon stimulation while simultaneously collecting a two photon image
• 2-Channel Non-Descanned Detectorv