Full Animal
Imaging
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IV100
Intravital Laser Scanning Microscope
The IV100 is a new intravital laser scanning microscope for small animal imaging. The core technology of IV100 is a new class of objective lens. Olympus has miniaturized the high performance objective to produce the MicroProbe objective lens, nicknamed the “Stick Lens” by researchers. With UIS2 technology, the performance of MicroProbe lenses are extended into the NIR range (up to 1000nm).
The IV100 is equipped with up to four lasers (488/561/633/748nm) and is able to image multiple fluorescence probes simultaneously. The scan head has a tilting capability to facilitate objective orientation in three dimensions. Three magnifications of MicroProbe lenses enable the user to capture either a large field of view or high resolution images on a living animal. Certain conventional microscope objectives can also be used on IV100 as well.
Features and Benefits
- 3 simultaneous detection channels for multicolor fluorescence
- 4 lasers 488, 561, 633, 748 nm
- Immersible MicroProbe lens technology for minimal invasiveness and chemical sterilization
- Multipositional, tilting scanhead
- X,Y,Z,T scan combination
- 76mm range in Z
- Heating plate and anesthesia device
Optics
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Name
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Diameter (mm)
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Field of View (mm)
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Working Distance (mm)
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NA
|
Mag.
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Length (mm)
|
Immersion
|
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IV-OB35F22W20
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3.5
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0.22
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0.20
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0.7
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27X
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25
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Water
|
|
IV-OB13F20W20
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1.3
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0.20
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0.20
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0.5
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20X
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20
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Water
|
|
IV-OB13F67W20
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1.3
|
0.67
|
0.20
|
0.14
|
6X
|
20
|
Water
|
The transmission and correction of the objective are up to 1000 nm.
Specifications
| IV100 Specifications |
| Laser Light Source |
Visable light laser |
Ar laser (488nm), DPSS 561 laser (561nm), HeNe(R) laser (633nm) Intensity control: Continuously variable through AOTF(0.1% — 100%, 0.1% steps) Automatic blanking in flyback motion REX: Capable of laser intensity adjustment and laser wavelength selection fm |
| Near-infrared laser |
LD748 laser (wavelength: 748m) |
| AOTF laser combiner |
Intensity and shutter control for each laser |
| Scanning unit |
Basic Configuration |
3 channels as standard. 3 photomultipliers. High performance 6-position filter turrets for emission beamsplitters and barrier filters. |
| Scanning mode |
2 galvanometer scanning mirrors |
| |
Pixel size: 64 X 64 — 4096 X 4096 |
| |
Scanning speed (512X512): approximately 0.25 s |
| |
Dimension: Time, Z |
| |
Line scanning: linear, free-line, point |
| Sensing method |
2 detection modes: Analog integration and hybrid photon counting |
| Field number |
13 |
| Zoom |
1X — 50X with 0.1X steps |
| Z-axis drive |
Uses motorized focusing module (minimum step: 0.01µm) |
| XY scanner positioning unit |
Motorized |
| Anti-vibration platform |
Built-in |
| PC with system control boards |
PC-AT compatible/OS: Windows XP Professional (English version)/memory: 1GB or larger CPU: Pentium 4, 2GHz or higher/ Hard disk: 80GB or larger Special Interface board (built-in PCI) Graphics board: Compliant with Open GL Recording medium: Built-in CD-R/RW |
| Anesthesia |
Isoflurane anesthesia with induction chamber and gas regulator included |
| Power supply |
Microscope: 6A, Scanning unit: 6.2A, Computer: 4.5A, Multi Ar laser: 10A, HeNe (R) laser: 0.5A, DPSS561 laser: 0.5A |
Notice: The IV100 intravital laser scanning microscope has many potential
applications. Certain of these applications and materials used with them may require
licensing under patents held by third parties. For example, AntiCancer, Inc
(http://www.anticancer.com). and Xenogen Corporation (http://www.xenogen.com)
each own several patents in the field of imaging cells genetically engineered to produce
light-emitting compounds. Olympus has obtained a license from AntiCancer, such that
non-profit entities purchasing the IV100 are licensed under AntiCancer U.S. Patent Nos.
6,232,523, 6,649,159, 6,235,968, 6,251,384, and 6,759,038 for non-commercial uses.
Olympus' sale or other transfer of the IV100 imaging system does not convey any
additional rights or licenses under third party patents. It is suggested that users of the
IV100 imaging system consult with counsel to determine whether licensing of additional
third party patents is required.
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